IP antibody use：3ug Flag Mouse IgG1 per ml Lysate，WB 1:5000

1、untransfected 293 cell lysate

2、transfected 293 cell lysate with C-Myc-tag fusion protein

3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)

4、IP (transfected 293+anti- C-Myc mAb+ Protein G agarose)

5、IP (transfected 293+Protein G)

6、Recombinant protein (E.coli)

1ug C-Myc fusion protein+ Primary antibody dilution at

1、1:5,000

2、1:10,000

IF analysis of 293 cells transfected with a C-Myc-tag protein, using TDYbio anti-C-MycTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-C-Myc)

Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

The C-Myc tag antibody can detect C-Myc fusion proteins.

Store at -20°C. Stable for one year from the date of shipment.

1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

WB 1:5,000 IP 1:200 IF 1:1,000

(Optimal dilutions should be determined by the end user)