010-80117836
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| β-tubulin Mouse mAb(5G3) |
| beta tubulin antibody, TUBB antibody |
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| Cat#： TDY043 | Source： Mouse | Type： Monoclonal | |
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| Applications: WB, IHC, IF |
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Reactivity: H, R, M, Mk, Dg, Ch, Hm, Rb, Sh, Pg, Insect, Yeast |
| MW.(KDa): 55 |
Isotype: IgG1 |
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Applications: WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
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| Western Blot |
Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.
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| Immunohistochemistry |
IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
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| IF |
IF analysis of Hela with TDY043(Left) and DAPI (Right) diluted at 1:100.
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| Source |
Mouse monoclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
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| Specificity |
The β-tubulin mouse monoclonal antibody can detect endogenous β-tubulin proteins.
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| Storage |
Store at -20°C. Stable for one year from the date of shipment.
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| Concentration |
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
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| Dilutions |
WB 1:5,000 IHC 1:200 IF 1:100-200
(Optimal dilutions should be determined by the end user)
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beta tubulin antibody, TUBB antibody |
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| Size: |
TDY043C-50μL ￥800
TDY043F-100μL ￥1500 |
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| New Product: |
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